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Flame testing

Submitted by sat on 02 May 2016

Answer edited 26/07/2016 to include link to the recently developed SOP: SOP: Demonstrating the flame test using a PET bottle and video presentation.

In brief

There are a number of different methods that could be used to show the colours of various cations, each having its own risks. These risks include handling hazardous chemicals or chemical solutions. The various cations that can be observed include: lithium, calcium, sodium, barium, copper and potassium, usually in the form of their chloride salts. Some of these are classified as having significant health hazards or acute toxicity, so careful handling is required. Methods that generate significant, uncontained levels of aerosols in the laboratory are not recommended, and in particular, we do not recommend the spraying of the solution into the flame for student activities. We advise against methods using flammable liquids.1

Any method employed requires a site-specific “risk assessment” to be performed. This should take into account the skills and behaviour of the students, as well as the learning outcome, to decide which method is considered most appropriate and whether to conduct the flame test as a teacher demonstration or a student activity.

If this activity is to engage the interest of students, then we suggest that a teacher demonstration may be the most appropriate approach for this activity for Year 8 students, with a student activity perhaps better suited for more meaningful curriculum applications in later years.

Different methods

Below are some methods that could be considered; they are listed in order, with the first being the preferred method, due to containment of the chemicals used. It is suggested that the different chemicals are set up in different stations around the classroom for methods 1–3, to minimise the opportunity for cross-contamination.

1. Flame test using PET bottle method.

Science ASSIST has developed an SOP and an accompanying video demonstration which describes this method, see SOP: Demonstrating the flame test using a PET bottle and video presentation. In brief, a Bunsen burner is fitted into the neck of a 2 L soft drink bottle with a hole for the Bunsen tubing and another hole for a garden spray bottle, which contains the aqueous test solution (~0.5–1.0 M). A fine mist is sprayed from the spray bottle into the air intake of the Bunsen burner. The colour of the flame changes to the characteristic colour of the cation in the spray bottle.

The advantage of this method is that the majority of the sprayed cation solution (many of which contain 'heavy metals') is contained within the experimental setup, minimising the spread and inhalation of aerosols in the laboratory environment. It also provides for the containment and collection of any overspray, which can be collected and reused. It also produces a longer-lasting colour in the flame for observation with the naked eye or with a spectroscope.

2. Flame test using wooden paddle-pop sticks/toothpicks (or similar)

  1. Wooden paddle-pop sticks or toothpicks are soaked in distilled or deionised water overnight. The following day, a stick is removed and dipped into the solid chemical to be tested. It is then passed through a blue Bunsen flame allowing the solid to burn and produce a colour change in the flame. A different stick is used for each chemical.
  2. Wooden paddle-pop sticks or toothpicks are soaked overnight to a depth of about 1cm of the stick in a concentrated (1–2 M) aqueous solution of the cation to be tested. The following day, the sticks are removed and passed through a blue Bunsen flame and the flame colour observed.

Disadvantages of these methods.

3. Flame test using a wire loop method

An inoculating loop made from either platinum or nichrome wire is generally used. The metal loop is first cleaned by dipping into either concentrated or high molarity HCl followed by rinsing in deionised water. The cleaned loop is then dipped either into the solid chemical or a solution of the chemical to be tested and then passed through the blue flame of a Bunsen burner. The flame colour is observed.

Disadvantages of this method

4. Flame test spraying directly into a Bunsen flame with a spray bottle

A fine mist of the test chemical in an aqueous solution is sprayed directly into a blue Bunsen flame from a spray bottle and any colour change observed. This is the least recommended method and should only be conducted as a teacher demonstration.

Disadvantages of this method.


1 ‘Safety Alert Rainbow Demonstration’ American Chemical Society website. (Accessed April 2016)

‘Barium chloride’ Safety Data Sheet. Chem Supply website, (February 2014)

‘Calcium chloride’ Safety Data Sheet. Please search the product information page on the Chem Supply website for the latest version: 

‘Copper (II) chloride’ Safety Data Sheet. Chem Supply website, (August 2013)

‘Lithium chloride’ Safety Data Sheet. Chem Supply website, (August 2015)

‘Potassium chloride’ Safety Data Sheet. Chem Supply website, (November 2015)

‘Sodium chloride’ Safety Data Sheet. Chem Supply website, (May 2013)

Flame testing

Submitted by nehal.trivediasta on 26 July 2016

Dear Teresa,

Thanks for the answer to the question. The answer will be definitely helpful to lot of users as it is very common experiment in chemistry.

Can you please provide the link to the Science ASSIST SOP for the same in the answer if ready and available for use?


With regards

Nehal Trivedi