Cleaning microscope slides
Cleaning microscope slides: What is the most effective way to clean glass microscope slides so they are suitable to reuse?
What is the usual practice for cleaning prepared slides?
Cleaning microscope slides: What is the most effective way to clean glass microscope slides so they are suitable to reuse?
What is the usual practice for cleaning prepared slides?
Submitted by sat on 12 April 2018
Answer reviewed 27 February 2023
Microscope slides are best handled carefully by holding the edges. Finger marks and other artefacts such as dust and scratches can affect the quality of the image viewed on the slide. Slides that are scratched should be disposed of into a glass/sharps bin
Caution: Methylated spirit is flammable1. Science ASSIST advises that glass slides not be stored in methylated spirit in school teaching laboratories.
New slides may have dirt or grease on them. To remove: follow procedure as for used slides outlined above (steps 3-8).
Heat fixed smears are difficult to clean so are not reused and should be disposed of with broken glass into a glass/sharps bin.
Low-lint tissue (Kimwipes®) is available from Science Suppliers. See our list of School science suppliers.
1 ChemSupplyAustralia. (2021). ChemSupplyAustralia business unit, Search Results, Methylated Spirits (95% IMS). Retrieved from ChemSupplyAustralia: https://shop.chemsupply.com.au/search?ProductSearch=methylated%20spirit
Simulab Testing Laboratory, LabTech Training. (n.d.). Cleaning and Handling Microscope Slides. Retrieved from Study Notes: http://simulab.ltt.com.au/4/laboratory/studynotes/SNCleanMicroSlides.htm