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Submitted by sat on 17 April 2019

Biodiesel is a renewable fuel and seen as an alternative to petroleum diesel.1 Biodiesel is produced by reacting an alcohol, usually methanol, with a vegetable oil or animal fat in the presence of a catalyst. Commonly used catalysts include potassium hydroxide or sodium hydroxide. The chemical reaction is called transesterification and the products formed are methyl esters (the biodiesel) and glycerol. There are several possible methods for making biodiesel in the school science laboratory.

We recommend the method and scale available through the BBSRC2 based upon the one published by CLEAPSS3, for the following reasons:

We recommend that if an extension activity for burning the biodiesel is to be conducted, that one of the following methods are used in a fume cupboard.


Waste Disposal

The transesterification reaction, which is used to produce biodiesel, produces methyl esters of fatty acids (the biodiesel), with glycerol as the main by-product. The reaction mixture will form two layers. The top layer contains the biodiesel as well as some unreacted vegetable oil (triacylglycerols), some methanol, glycerol and other contaminants. The bottom layer contains glycerol, methanol, methoxide salt, soap (saponified fatty acids) and hydroxide. The ratio of biodiesel to glycerol in the product is about 10:1 by mass.

There are methods to treat both the biodiesel and the glycerol by-product for disposal, however these are generally time consuming and not practical for the school setting.6

Unused biodiesel must be kept for a licenced chemical waste disposal contractor because of the contaminants in it. It can be combined with other non-halogenated organic waste. Whether kept separate or combined, a label should be affixed on the side of the bottle stating the contents.

If the reaction is carried out on a small scale so that only very small amounts of glycerol are produced, then the glycerol layer can be washed down the sink. Glycerol is miscible with water and biodegradable, however it has a high oxygen demand (i.e. oxygen in the water body will be consumed as the glycerol degrades) and so, larger quantities should not be washed to waste. Larger quantities of glycerol should be stored for collection by a licenced waste disposal contractor.

References and further reading

1‘What is biodiesel?’, Bioenergy Australia website, (Link updated October 2019)

2MacLean, Tristan. 2014. Practical Biofuel Activities for School Engagement and Outreach, Activity 1D Biodiesel production pp25–30, Biotechnology and Biological Sciences Research Council (BBSRC). Available for download at ‘Practical biofuel activities’, Biotechnology and Biological Sciences Research Council (BBSRC) website, (Accessed April 2019)

3CLEAPSS. 2007. Making biodiesel, CLEAPSS website, (Login required)

4 ‘The preparation of biodiesel from rape seed oil – or other suitable vegetable oils worksheet’,Learn Chemistry, RSC website, (Accessed April 2019)

5‘Preparation & Combustion of Biodiesel’, American Chemical Society website, (Accessed April 2019)

6 ‘Biodiesel Safety and Best Management Practices for Small-Scale Noncommercial Use and Production’,Sustainable Agriculture Research and Education (SARE) website, (2008)


Australian Government, Department of the Environment, Water, Heritage and the Arts. n.d. Why we should recycle used motor oil, Australian Government, Department of the Environment, Water, Heritage and the Arts website, (Accessed April 2019)

‘Biodiesel part 1 – Teacher Notes, Biodiesel part 1 – Student Notes, Biodiesel part 2 – Teacher Notes, ‘Biodiesel part 2 – Student Notes’, Chemical Education Group webpage, Royal Australian Chemical Institute website. (Accessed April 2019. Link no longer available July 2020)

‘Biofuel Chemistry: How they Burn?’, Biofuel UK website (Accessed April 2019)

‘Fuels’, Q&A Science ASSIST website, (September 2015)

‘Toxic alcohols in spirit burners to test fuel efficiency’, Q&A, Science ASSIST website, (June 2016)

Traviss N. ‘Breathing easier? The known impacts of biodiesel on air quality’, Biofuels, 2012;3(3):285–291. doi:10.4155/bfs.12.22 (See the executive summary at the end on biodiesel vs diesel)

‘Waste Management in Biodiesel Production’, eXtension website, (26 March 2012)

‘What are biofuels?’, Bioenergy Australia website, (Link updated October 2019)