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Answer by labsupport on question Genetic modification of bacteria

Submitted by sat on 13 March 2019

Regulation

The Office of the Gene Technology Regulator website has the legal information regarding the regulation of genetically modified organisms, see http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/Content/factsheets. A link to a Fact Sheet - GM kits in Schools is provided on this site and contains the information that you require.

Suitability of this activity for schools

It is important to be aware of the risks and safety issues regarding the microbiological aspects of these kits and to confirm if the required techniques and procedures are allowed in your school jurisdiction.

If your school jurisdiction allows, then this activity is permissible provided that a site-specific biological risk assessment has been conducted and approved by your school. (See below). Detailed information for this activity has been provided in a previous Q&A Transformation of E.coli with pFluoroGreen, some of which is repeated below.

Subculturing – This activity requires E.coli cultures to be subcultured from both solid and liquid media. The specialised technique of subculturing requires sound knowledge and expertise to minimise risks involved. Many jurisdictions do not allow cultures to be opened for any manipulation due to the significant risks of contamination and growing unknown microorganisms.

Incubation temperature – This activity requires growth at 370C as the E.coli will not grow well at temperatures below this. School jurisdictions recommend incubation of microorganisms at room temperature up to a maximum of 300C and not at 370C to minimise the likelihood for growth of human pathogens.

Selective media – The use of simple media such as nutrient agar is recommended for use in schools. Selective or enriched media which encourages the growth of pathogens is not advised for use in schools.

Use of penicillin – This activity requires the addition of an antibiotic (ampicillin) to agar, which produces a selective medium for the growth of E.coli which contains the gene for ampicillin resistance. Ampicillin is also a member of the penicillin family of antibiotics and staff or students who may be allergic to penicillin should avoid all contact.

Sterilisation and decontamination – The genetically modified bacteria produced need to be killed and not released into the environment. The use of an autoclave is the method of choice to decontaminate microbial cultures and waste. This activity suggests using 10% bleach (a disinfectant) as an alternative if an autoclave is not available. Science ASSIST does not recommend the use of 10% bleach (chemical sterilisation) as it is an unsatisfactory alternative to autoclaving. It has a number of limitations and is less effective against spores.

Other microbiology considerations

There are many other aspects to conducting microbiology in schools that must be considered before proceeding.

Firstly, schools must ensure that they have the required facilities and equipment and the necessary staff training to be able to manage the risks of microbiological work.

Secondly, schools should choose the microorganism with the lowest level of risk that will meet the learning outcomes.

Thirdly, before, schools embark on working with microorganisms they should ask the following questions and perform a site-specific biological risk assessment.

Science ASSIST has produced GUIDELINES for best practice for microbiology in Australian schools. See https://assist.asta.edu.au/resource/4196/guidelines-best-practice-microbiology-australian-schools

We recommend that your school is familiar with the content of this guide before contemplating the delivery of practical activities in microbiology. In particular, see pp 12-13 for section 3.1 Risk assessment.

We have previously answered the following related questions, which can be viewed on our website:

Using E. coli bacteria in schools

Gene induction experiment?

Transformation of E.coli with pFluoroGreen

References and further reading:

‘Activity 4: Transformation of E. coli using green fluorescent protein’,  The American Phytopathological Society website, https://www.apsnet.org/EDCENTER/K-12/TEACHERSGUIDE/PLANTBIOTECHNOLOGY/Pa...(Accessed March 2019)

‘Fact sheets’, Office of the Gene Technology Regulator website, http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/Content/factsheets (27 September 2018)

Science ASSIST. 2017. GUIDELINES for best practice for microbiology in Australian schools, Science ASSIST website, https://assist.asta.edu.au/resource/4196/guidelines-best-practice-microbiology-australian-schools