Microbiology (cultivating temperatures)

Microbiology (cultivating temperatures): Hi, We are interested in buying a genetic modifying kit from BioRad.  Website: http://www.bio-rad.com/en-au/category/pglo-plasmid-gfp-kits

I understand this procedure is allowed in schools (please correct me if I'm wrong), but it requires the plates to be grown at 37° C.  From my understanding—I can't find it written, hence my query—I thought plates were not allowed to be grown at 37° C because they could grow pathogenic organisms. What temperature are plates allowed to be grown at in a school environment?

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Publication Date: 31 August 2015
Asked By: Glenda
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Microbiology

Science ASSIST recommends a risk assessment be conducted prior to the purchase and use of all biological materials so that all hazards can be identified and appropriate control measures implemented. Science ASSIST has developed some resources to help in this process, see AIS: Risk Management and risk assessment  Risk Assessment Template.


Regarding the temperature at which agar plates should be grown in a school environment:


You are correct that microorganisms should not be incubated at 37° C.  The recommended temperature for the incubation of microorganisms in schools is at room temperature or up to a maximum of 30 °C to minimise the likelihood for growth of potential human pathogens that are adapted to human body temperature.[i] Science ASSIST advises against the incubation of microorganisms at 37 °C.


Regarding the use of the genetic modifying kit being used in schools:


Science ASSIST is seeking further clarification on various aspects of this activity and will provide a detailed response as soon as possible.



[i] Society for General Microbiology (SGM). 2006. Basic Practical Microbiology – A Manual. Reading UK. http://www.microbiologyonline.org.uk/file/ca2189fba3b39d24c5a44c1285d008...

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