Method for testing pH and catalase in liver for Yr 12 biology

Method for testing pH and catalase in liver for Yr 12 biology: I am looking for materials and method for a practical that tests pH and catalase in liver for year 12 biology.

The idea is to use detergent to measure foam levels, buffer solutions and universal indicator with chopped up liver using a range of pH. (optimal level is 7). Do you have anything, or can you make a list that I could give to our lab technician?

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Publication Date: 13 February 2019
Asked By: Anonymous
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Answer by labsupport on question pH and enzymes (catalase in liver)

The Biology Corner website has an investigation on enzymes (https://www.biologycorner.com/worksheets/enzyme_lab.html) that is well set out and may be suitable for your needs. It does not include the use of detergent; however, you could add universal indicator and some detergent to Part D to achieve the outcome you are looking for. As with all new activities, it is recommended that you and/or your technician trial the activity to ensure that it suits your purposes and is workable.

A few other links that may be helpful are listed below:

‘Factors affecting enzyme activity’, Nuffield Foundation website, http://www.nuffieldfoundation.org/practical-biology/factors-affecting-en... (Accessed February 2019) (some examples of a range of different enzymes and activities)

'Liver Stinks!’, Science Buddies website, https://www.sciencebuddies.org/science-fair-projects/project-ideas/BioCh... (28 July 2017) (an activity testing under different conditions without test tubes)

‘Practical Enzyme Experiments Anyone Can Do’, Practical Biology: science for everyone website, http://practicalbio.blogspot.com/2012/11/easy-enzyme-experiments-anyone-... (November 2012) (a blog on enzymes including 2 activities using the catalase enzyme)

 ‘The Effect of Hydrogen Peroxide on Liver: Hypothesis, Apparatus, Method’, Schoolworkhelper website, https://schoolworkhelper.net/the-effect-of-hydrogen-peroxide-on-liver-hy... (2017) (some general information)

 ‘The Liver: Helping Enzymes Help You!’, Scientific American website, https://www.scientificamerican.com/article/bring-science-home-liver-help... (8 March 2012) (an activity testing under different conditions without test tubes)

Method for testing pH and catalase in liver for Yr 12 biology

 

Rather  than using liver, consider using grated potato. Method:  place  2g of freshly grated potato into a25 or 50ml measuring cylinder. Add 2-5ml of water or buffer depending on your variable. Add a drop or two of detergent and Mix . Add 1ml 3% hydrogen peroxide.  Read the height of the foam column after a set time. potato is much nicer to clean up than liver especially if temperature is your variable.

 

 

 

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