Aceto-orcein stain is an effective procedure for the visualisation of mitosis in root tips of onions or garlic. Orcein can be purchased in solid form, the solution is prepared with acetic acid. A full method for stain preparation and procedure can be found at Mitosis in Growing Root Tips1.
Alternatively, Aceto-orcein can be purchased as a 1% solution to be used undiluted2. Each student group requires one drop of stain, so the prepared stain is cost effective. The cost of the stain and delivery charges vary between suppliers, and is moderately priced. Refer to our School science suppliers list for suppliers of biological chemicals. Aceto-orcein solution is suitable for laboratory use for students in Years 11 and 123.
Alternative stains: The following stains will also show mitosis in root tips. The cost of these stains is similar to Orcein. Methylene Blue and Toluidine Blue stains are relatively quick to prepare, and staining procedures are similar in each.
- Toluidine blue: http://www.gtac.edu.au/wp-content/uploads/2016/01/Mitosis_Roottips_LabPr...
- Methylene blue: https://www.flinnsci.com/making-mitosis-slides/dc10945/
- Shiffs Reagent (also known as Feulgen stain4): http://w3.marietta.edu/~biol/introlab/Onion%20root%20mitosis.pdf Whilst the preparation of this reagent is more complicated, this procedure does include a good method for softening the roots, by using a micro-tube placed in a water bath at 60°C.
Science ASSIST recommends you consult the relevant Safety Data Sheet first and our List of recommended chemicals for science in Australian schools to ascertain the suitability of these stains for your staff and students.
1 ‘Mitosis in growing root tips’. Gene Technology Access Centre website. http://www.gtac.edu.au/wp-content/uploads/2016/01/Mitosis_Roottips_LabPr... (accessed August 2017)
2 ‘Aceto-orcein 1% SI1' product description. Southern Biological. http://www.southernbiological.com/chemicals/stains-and-indicators-soluti... (accessed August 2017)
3 Science ASSIST. 2016. List of recommended chemicals for science in Australian schools. https://assist.asta.edu.au/sites/assist.asta.edu.au/files/ListOfRecommen...
4 Reader S. February 2003. A modified method of preparing Feulgen stain or Shiff’s Reagent. https://web.archive.org/web/20130903160922/http://www.jic.ac.uk:80/staff... (The original resource is no longer available on this website, this copy made available by the Internet Archive, December 2018).